2CDU
The Crystal Structure of Water-forming NAD(P)H Oxidase from Lactobacillus sanfranciscensis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-03-14 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 59.636, 92.644, 163.465 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 81.650 - 1.800 |
R-factor | 0.18 |
Rwork | 0.178 |
R-free | 0.22300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1f8w |
RMSD bond length | 0.015 |
RMSD bond angle | 1.542 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.400 | 1.850 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.100 | 0.490 |
Number of reflections | 81507 | |
<I/σ(I)> | 29 | 1.9 |
Completeness [%] | 96.1 | 73.8 |
Redundancy | 8.5 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 10 MG/ML PROTEIN, 100MM HEPES PH 7.0, 18% W/V PEG 4000, 8% V/V 2-PROPANOL, 20 MM DITHIOTHREITOL |