2CAZ
ESCRT-I core
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-06 |
| Detector | ADSC CCD |
| Spacegroup name | P 61 |
| Unit cell lengths | 167.914, 167.914, 50.242 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 145.860 - 3.600 |
| R-factor | 0.329 |
| Rwork | 0.328 |
| R-free | 0.35700 |
| Structure solution method | MAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.280 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SnB |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.000 | 3.800 |
| High resolution limit [Å] | 3.600 | 3.600 |
| Rmerge | 0.060 | 0.400 |
| Number of reflections | 9610 | |
| <I/σ(I)> | 24.21 | 1.8 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.7 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 290 | 8-10% PEG 8000, 8-9% ETHYLENE GLYCOL, 0.1 M HEPES (PH 7.0- 8.0), 17 DEG C |
