2C9Z
Structure and activity of a flavonoid 3-0 glucosyltransferase reveals the basis for plant natural product modification
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2005-05-01 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.189, 93.817, 106.092 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.100 |
| R-factor | 0.2 |
| Rwork | 0.198 |
| R-free | 0.23500 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.309 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.110 | 0.410 |
| Number of reflections | 29411 | |
| <I/σ(I)> | 12.1 | 3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 4.4 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 20% PEG 10000, 0.1M BIS-TRIS PROPANE PH 7.0, 0.5% PRUCORONIC F-68 |
