2C9U
1.24 Angstroms resolution structure of as-isolated Cu-Zn Human Superoxide dismutase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX10.1 |
| Synchrotron site | SRS |
| Beamline | PX10.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-07-10 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.638, 67.553, 52.320 |
| Unit cell angles | 90.00, 106.48, 90.00 |
Refinement procedure
| Resolution | 50.190 - 1.240 |
| R-factor | 0.141 |
| Rwork | 0.139 |
| R-free | 0.17600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hl5 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.841 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.280 |
| High resolution limit [Å] | 1.240 | 1.240 |
| Rmerge | 0.060 | 0.400 |
| Number of reflections | 70045 | |
| <I/σ(I)> | 20 | 2.2 |
| Completeness [%] | 96.3 | 92.5 |
| Redundancy | 3.5 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 3.0 M AMMONIUM SULPHATE, 100 MM SODIUM CHLORITE, 50MM SODIUM ACETATE BUFFER, pH 8.00 |
