2C9R
apo-H91F CopC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | I 41 |
| Unit cell lengths | 72.315, 72.315, 47.542 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.740 - 2.000 |
| R-factor | 0.177 |
| Rwork | 0.175 |
| R-free | 0.21300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2c9q |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.384 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.040 | 0.240 |
| Number of reflections | 8189 | |
| <I/σ(I)> | 23.8 | 3.7 |
| Completeness [%] | 97.7 | 95.6 |
| Redundancy | 2.4 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.6 | 2.4 M SODIUM ACETATE, PH 7.0, 0.1 M BIS-TRIS PROPANE, PH 6.6, EDTA, 10 MM. |
