2C9O
3D Structure of the human RuvB-like helicase RuvBL1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 6 |
| Unit cell lengths | 207.080, 207.080, 60.710 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 179.610 - 2.200 |
| R-factor | 0.209 |
| Rwork | 0.206 |
| R-free | 0.25700 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.500 |
| Data scaling software | SCALA |
| Phasing software | SHELXCD |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.400 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.070 | 0.620 |
| Number of reflections | 73593 | |
| <I/σ(I)> | 8.1 | 1.2 |
| Completeness [%] | 96.5 | 80 |
| Redundancy | 8.3 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | PROTEIN WAS CRYSTALLISED USING THE VAPOR DIFFUSION METHOD (SITTING DROP). THE WELLS CONTAINED 500 MICROLITERS OF PRECIPITANT SOLUTION AND THE DROPS WERE COMPOSED OF 1.5 MICROLITERS OF PROTEIN SOLUTION (15 MG/ML) AND 1.5 MICROLITERS OF RESERVOIR SOLUTION. THE BEST CRYSTALS WERE OBTAINED FROM A SOLUTION CONTAINING 1.6 M SODIUM MALONATE PH 6 AT 293 K. |
