2C9O

3D Structure of the human RuvB-like helicase RuvBL1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 6
Unit cell lengths	207.080, 207.080, 60.710
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	179.610 - 2.200
R-factor	0.209
Rwork	0.206
R-free	0.25700
Structure solution method	SAD
RMSD bond length	0.014
RMSD bond angle	1.500
Data scaling software	SCALA
Phasing software	SHELXCD
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.400	2.320
High resolution limit [Å]	2.200	2.200
Rmerge	0.070	0.620
Number of reflections	73593
<I/σ(I)>	8.1	1.2
Completeness [%]	96.5	80
Redundancy	8.3	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	PROTEIN WAS CRYSTALLISED USING THE VAPOR DIFFUSION METHOD (SITTING DROP). THE WELLS CONTAINED 500 MICROLITERS OF PRECIPITANT SOLUTION AND THE DROPS WERE COMPOSED OF 1.5 MICROLITERS OF PROTEIN SOLUTION (15 MG/ML) AND 1.5 MICROLITERS OF RESERVOIR SOLUTION. THE BEST CRYSTALS WERE OBTAINED FROM A SOLUTION CONTAINING 1.6 M SODIUM MALONATE PH 6 AT 293 K.