2C91
mouse succinic semialdehyde reductase, AKR7A5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-24 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 98.532, 159.238, 96.698 |
| Unit cell angles | 90.02, 119.40, 78.50 |
Refinement procedure
| Resolution | 45.000 - 2.300 |
| R-factor | 0.162 |
| Rwork | 0.160 |
| R-free | 0.20800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gve CHAIN A |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.889 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.110 | 0.890 |
| Number of reflections | 630867 | |
| <I/σ(I)> | 4.1 | 1 |
| Completeness [%] | 98.4 | 93.3 |
| Redundancy | 2.9 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | SITTING DROP METHOD EQUILIBRATION OF 15 MG/ML AKR7A5, 20MM TRIS-CL PH7.5, 0.5M DITHITHREITOL, 1MM NADP AGAINST 0.2M SODIUM TARTRATE, 7.5% PEG8000 AND 0.1M MES-NAOH PH6.5, pH 6.50 |
