2C8V
Insights into the role of nucleotide-dependent conformational change in nitrogenase catalysis: Structural characterization of the nitrogenase Fe protein Leu127 deletion variant with bound MgATP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2002-12-20 |
| Detector | MARRESEARCH |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 70.900, 133.300, 61.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.500 |
| R-factor | 0.2381 |
| Rwork | 0.238 |
| R-free | 0.27930 |
| Structure solution method | OTHER |
| RMSD bond length | 0.010 |
| RMSD bond angle | 2.520 |
| Data reduction software | Blu-Ice |
| Data scaling software | MOSFLM |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.086 | 0.079 |
| Number of reflections | 20818 | |
| <I/σ(I)> | 4.1 | 5.1 |
| Completeness [%] | 97.8 | 99.9 |
| Redundancy | 4 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 24% POLY(ETHYLENE GLYCOL), MW 4000, 0.16 M MGCL2, TRISHYDROCHLORIC ACID, PH 8.5, 20% GLYCEROL, AND 1MM SODIUM DITHIONITE |
