2C8L
Crystal Structure of (SR) Calcium-ATPase E2(Tg) form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-27 |
| Detector | MARRESEACH |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 71.514, 71.514, 589.355 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.990 - 3.100 |
| R-factor | 0.249 |
| Rwork | 0.249 |
| R-free | 0.30600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1iwo |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.000 | 3.170 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.100 | 0.730 |
| Number of reflections | 29437 | |
| <I/σ(I)> | 20.8 | 2.79 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 6.7 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.8 | 50 MM NAOAC, 15% PEG2000 MME, 10& GLYCEROL, 4% MPD, pH 6.80 |
