2C81
Crystal structures of the PLP- and PMP-bound forms of BtrR, a dual functional aminotransferase involved in butirosin biosynthesis.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-01-08 |
| Detector | RIGAKU-MSC RAXIS IV |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 73.750, 73.750, 162.360 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.370 - 1.700 |
| R-factor | 0.189 |
| Rwork | 0.189 |
| R-free | 0.20900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2c7t |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.050 | 0.370 |
| Number of reflections | 52335 | |
| <I/σ(I)> | 10 | |
| Completeness [%] | 91.5 | 98.9 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1M NAHEPES, K,NA TARTRATE TETRAHYDRATE PH7.5 |
