2C6U
Crystal structure of human CLEC-2 (CLEC1B)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.1 |
| Synchrotron site | SRS |
| Beamline | PX14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-28 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 35.064, 55.065, 56.165 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.310 - 1.600 |
| R-factor | 0.161 |
| Rwork | 0.159 |
| R-free | 0.19800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | COMPOSITE MODEL - SEE REMARKS |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.187 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CaspR |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.490 |
| High resolution limit [Å] | 1.440 | 1.440 |
| Rmerge | 0.040 | 0.110 |
| Number of reflections | 20396 | |
| <I/σ(I)> | 49.02 | 10.13 |
| Completeness [%] | 88.0 | 24.4 |
| Redundancy | 11.4 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 20% W/V POLYETHYLENE GLYCOL MONOMETHYL ETHER 5000 0.1M BIS-TRIS PH 6.5 |
