2C6D

Aurora A kinase activated mutant (T287D) in complex with ADPNP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	100
Detector technology	CCD
Collection date	2001-01-30
Detector	ADSC CCD
Spacegroup name	P 32 2 1
Unit cell lengths	86.500, 86.500, 78.300
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.000 - 2.200
Rwork	0.230
R-free	0.28000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1atp
RMSD bond length	0.006
RMSD bond angle	1.300
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	CNX (2000)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.000	2.250
High resolution limit [Å]	2.200	2.200
Rmerge	0.040	0.330
Number of reflections	17003
<I/σ(I)>	19.3	3
Completeness [%]	97.1	83.2
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		3.8		pH 3.80