2C5L

Structure of PLC epsilon Ras association domain with hRas

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2005-09-07
Detector	ADSC CCD
Spacegroup name	P 21 21 21
Unit cell lengths	72.416, 93.618, 111.494
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	111.800 - 1.900
R-factor	0.187
Rwork	0.185
R-free	0.22800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.017
RMSD bond angle	1.521
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.870	2.820
High resolution limit [Å]	2.800	2.800
Rmerge	0.080	0.480
Number of reflections	18504
<I/σ(I)>	10.46	2.09
Completeness [%]	100.0	100
Redundancy	4.28	4.32

Crystallization Conditions

crystal ID	method	pH	temperature	details
1