2C5E
gdp-mannose-3', 5' -epimerase (arabidopsis thaliana), k217a, with gdp-alpha-d-mannose bound in the active site.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.481, 83.617, 66.232 |
| Unit cell angles | 90.00, 99.01, 90.00 |
Refinement procedure
| Resolution | 34.610 - 1.700 |
| R-factor | 0.134 |
| Rwork | 0.131 |
| R-free | 0.19300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2c54 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.735 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.220 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.090 | 0.440 |
| Number of reflections | 73777 | |
| <I/σ(I)> | 13.2 | 2.2 |
| Completeness [%] | 99.8 | 99.7 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 100 MM BIS-TRIS PH 6.0, 2.2 M AMMONIUM SULPHATE. SITTING DROP, VAPOUR DIFFUSION. CRYOPTROTECTED WITH 6 M SODIUM FORMATE. |
