2C53
A comparative study of uracil DNA glycosylases from human and herpes simplex virus type 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.406, 61.161, 43.620 |
| Unit cell angles | 90.00, 93.15, 90.00 |
Refinement procedure
| Resolution | 14.880 - 1.800 |
| R-factor | 0.164 |
| Rwork | 0.164 |
| R-free | 0.21600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.700 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.080 | 0.260 |
| Number of reflections | 19411 | |
| <I/σ(I)> | 13.83 | 2.27 |
| Completeness [%] | 93.7 | 63.1 |
| Redundancy | 3.3 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
