2C49

Crystal Structure of Methanocaldococcus jannaschii Nucleoside Kinase - An Archaeal Member of the Ribokinase Family

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 21 21 21
Unit cell lengths	64.006, 83.131, 146.784
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	72.550 - 1.920
R-factor	0.245
Rwork	0.243
R-free	0.28100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	1.700
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	1.930
Rmerge	0.060
Number of reflections	58526
<I/σ(I)>	20
Completeness [%]	97.0
Redundancy	13.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				15% PEG4000, 0.10 M MGCL2, 0.1 M TRIS-HCL PH 8.5