2C22
Efficient and High Fidelity Incorporation of dCTP Opposite 7,8- Dihydro-8-oxodeoxyguanosine by Sulfolobus solfataricus DNA Polymerase Dpo4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2005-08-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 93.277, 103.910, 52.498 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.470 - 2.560 |
| R-factor | 0.23 |
| Rwork | 0.230 |
| R-free | 0.26400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2br0 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.300 |
| Data reduction software | X-GEN |
| Data scaling software | X-GEN |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.470 | 2.720 |
| High resolution limit [Å] | 2.560 | 2.560 |
| Rmerge | 0.070 | |
| Number of reflections | 17101 | |
| <I/σ(I)> | 7.9 | |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 6.78 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 5% PEG 3350, 10 MM TRIS-HCL PH 7.5, 50 MM CA(CH3CO2)2, 30MM NACL, 1.25% GLYCEROL |
