2C1X
Structure and activity of a flavonoid 3-O glucosyltransferase reveals the basis for plant natural product modification
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-3 |
Synchrotron site | ESRF |
Beamline | ID14-3 |
Temperature [K] | 120 |
Detector technology | CCD |
Collection date | 2005-03-20 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 49.014, 93.394, 106.464 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.190 - 1.900 |
R-factor | 0.187 |
Rwork | 0.185 |
R-free | 0.22600 |
Structure solution method | SAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.515 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.190 | 1.950 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.090 | 0.470 |
Number of reflections | 37371 | |
<I/σ(I)> | 13 | 3.3 |
Completeness [%] | 100.0 | 100 |
Redundancy | 5.4 | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 20% PEG 10000, 0.1M BIS-TRIS PROPANE PH 7.0, 0.5% V/V PLURONIC ACID |