2C1L
Structure of the BfiI restriction endonuclease
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID13 |
| Synchrotron site | ESRF |
| Beamline | ID13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-09-09 |
| Detector | MARRESEARCH |
| Spacegroup name | I 4 |
| Unit cell lengths | 138.925, 138.925, 94.135 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.010 - 1.900 |
| R-factor | 0.188 |
| Rwork | 0.188 |
| R-free | 0.21700 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.600 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MLPHARE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.050 | 0.260 |
| Number of reflections | 70374 | |
| <I/σ(I)> | 10.4 | 2.7 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | MES 0.1M PH 6.5 K/NA TARTRATE 1.1M GLYCEROL 25% |
