2BZN
Crystal structure of human guanosine monophosphate reductase 2 GMPR2 in complex with IMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-28 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 131.291, 141.350, 164.895 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 51.370 - 2.150 |
| R-factor | 0.161 |
| Rwork | 0.158 |
| R-free | 0.21000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ble |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.512 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.260 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.060 | 0.290 |
| Number of reflections | 162865 | |
| <I/σ(I)> | 20.2 | 5.1 |
| Completeness [%] | 98.2 | 87.7 |
| Redundancy | 6.1 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.8 | pH 5.80 |
