2BYM
Histone fold heterodimer of the Chromatin Accessibility Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 130.550, 130.550, 59.710 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.960 - 2.800 |
| R-factor | 0.217 |
| Rwork | 0.217 |
| R-free | 0.27100 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.130 | 0.480 |
| Number of reflections | 13325 | |
| <I/σ(I)> | 5.6 | 1.6 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 14 | 14.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 12% PEG 3350, 0.1M HEPES PH 7.5, 5MM CDCL2 |
