2BYD
Structure of aminoadipate-semialdehyde dehydrogenase- phosphopantetheinyl transferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-03 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.780, 69.948, 71.239 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.940 - 2.000 |
| R-factor | 0.177 |
| Rwork | 0.173 |
| R-free | 0.24200 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.282 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.500 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.130 | 0.580 |
| Number of reflections | 21391 | |
| <I/σ(I)> | 11.8 | 2.27 |
| Completeness [%] | 96.4 | 77.6 |
| Redundancy | 6.6 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.7 | 0.05 M H3CIT/NA3CIT PH=5.7 14% PEG3350, pH 5.70 |
