2BX5

Is FR1 the antibody's Achillies heel

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Temperature [K]	100
Spacegroup name	P 64 2 2
Unit cell lengths	191.928, 191.928, 197.439
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	166.000 - 2.700
R-factor	0.25
Rwork	0.250
R-free	0.29000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hez
Phasing software	PHASER
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	166.000	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.090	0.420
Number of reflections	59210
<I/σ(I)>	8.5	2
Completeness [%]	91.4	89.7
Redundancy	2.3	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1