2BV8
The crystal structure of Phycocyanin from Gracilaria chilensis.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2001-12-10 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 101.985, 151.803, 101.548 |
| Unit cell angles | 90.00, 117.45, 90.00 |
Refinement procedure
| Resolution | 52.830 - 2.010 |
| R-factor | 0.1994 |
| Rwork | 0.199 |
| R-free | 0.23060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gh0 |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.300 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 2.120 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.060 | 0.300 |
| Number of reflections | 172985 | |
| <I/σ(I)> | 18.87 | 5.78 |
| Completeness [%] | 93.6 | 80.4 |
| Redundancy | 3.5 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | VAPOUR DIFFUSION, HANGING DROP, 18 MG/ML PROTEIN CONC., 1 M AM. SULPHATE, 50 MM PHOSPHATE PH7, PH 7.00 |
