2BV4
1.0A Structure of Chromobacterium Violaceum Lectin in Complex with alpha-methyl-mannoside
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-07-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 50.685, 89.504, 46.484 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.470 - 1.000 |
| R-factor | 0.107 |
| Rwork | 0.107 |
| R-free | 0.12300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gzt |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.745 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | ACORN |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.240 | 1.030 |
| High resolution limit [Å] | 1.000 | 1.000 |
| Rmerge | 0.060 | 0.440 |
| Number of reflections | 114226 | |
| <I/σ(I)> | 6.48 | 1.67 |
| Completeness [%] | 99.6 | 94.4 |
| Redundancy | 7.02 | 3.04 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | PEG 8000 10%, 0.1M NACL |
