2BTY
Acetylglutamate kinase from Thermotoga maritima complexed with its inhibitor arginine
Replaces: 1UVVExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-10-22 |
| Detector | ADSC CCD |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 94.998, 207.709, 117.996 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.950 - 2.750 |
| R-factor | 0.242 |
| Rwork | 0.242 |
| R-free | 0.27300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1buf |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.700 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.850 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.060 | 0.360 |
| Number of reflections | 30394 | |
| <I/σ(I)> | 22 | 4.1 |
| Completeness [%] | 98.8 | 98.4 |
| Redundancy | 3.2 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 15% POLYETHYLENE GLYCOL 4K, SODIUM ACETATE 50 MM PH 4.6, AMMONIUM SULFATE 75 MM |
