2BTM
DOES THE HIS12-LYS13 PAIR PLAY A ROLE IN THE ADAPTATION OF THERMOPHILIC TIMS TO HIGH TEMPERATURES?
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.5 |
| Synchrotron site | SRS |
| Beamline | PX9.5 |
| Temperature [K] | 297 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-03-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 78.132, 107.913, 70.980 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.400 |
| R-factor | 0.1756 |
| Rwork | 0.176 |
| R-free | 0.22000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1btm |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.253 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.4A) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.073 | 0.278 |
| Total number of observations | 70035 * | |
| Number of reflections | 22732 | |
| Completeness [%] | 94.3 | 65 |
| Redundancy | 3.1 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 | 20 * | pH 6.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7 (mg/ml) | |
| 10 | 1 | reservoir | sodium azide | 1 (mM) | |
| 11 | 1 | reservoir | dithiothreitol | 2 (mM) | |
| 2 | 1 | drop | MES | 5 (mM) | |
| 3 | 1 | drop | EDTA | 1 (mM) | |
| 4 | 1 | drop | sodium azide | 1 (mM) | |
| 5 | 1 | drop | 2-phosphoglycolate | 2 (mM) | |
| 6 | 1 | reservoir | PEG4000 | 25 (%(w/v)) | |
| 7 | 1 | reservoir | isopropyl alcohol | 8 (%) | |
| 8 | 1 | reservoir | acetate | 100 (mM) | |
| 9 | 1 | reservoir | EDTA | 1 (mM) |
