2BTF
THE STRUCTURE OF CRYSTALLINE PROFILIN-BETA-ACTIN
Experimental procedure
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.950, 71.300, 171.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 * - 2.550 |
| R-factor | 0.199 |
| Rwork | 0.199 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 3.800 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.550 * |
| Rmerge | 0.065 * |
| Number of reflections | 16158 * |
| Completeness [%] | 98.8 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4 * | referred to 'Segura, M. & Lindberg, U.', (1984) J.Biol.Chem., 259, 3949-3954 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | 150 (mM) | ||
| 2 | 1 | reservoir | 2 (mM) | ||
| 3 | 1 | reservoir | ATP | 0.5 (mM) | |
| 4 | 1 | reservoir | DTT | 0.5 (mM) |
