2BT9
Lectin from Ralstonia solanacearum complexed with Me-fucoside
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-01-31 |
| Detector | ADSC CCD |
| Spacegroup name | P 31 |
| Unit cell lengths | 43.985, 43.985, 103.076 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.000 - 0.940 |
| R-free | 0.12010 |
| Structure solution method | OTHER |
| RMSD bond length | 0.014 |
| RMSD bond angle | 0.030 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.670 | 0.960 |
| High resolution limit [Å] | 0.940 | 0.940 |
| Rmerge | 0.063 | 0.380 |
| Number of reflections | 142705 | |
| <I/σ(I)> | 15.32 | 1.28 |
| Completeness [%] | 98.3 | 79.5 |
| Redundancy | 5.79 | 3.68 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | HANGING DROPS WITH 2.5 UL RSL AT 15MG/ML, 2 UL OF RESERVOIR SOLUTION (30% PEG 6000, 0.1M TRIS/HCL PH 8.2) AND 1 UL OF GLYCINE. |
