2BT3
AGAO in complex with Ruthenium-C4-wire at 1.73 angstroms
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-01-01 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 158.059, 62.905, 92.097 |
| Unit cell angles | 90.00, 112.11, 90.00 |
Refinement procedure
| Resolution | 15.020 - 1.730 |
| R-factor | 0.155 |
| Rwork | 0.154 |
| R-free | 0.17100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1w6g |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.529 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.760 |
| High resolution limit [Å] | 1.730 | 1.730 |
| Rmerge | 0.040 | 0.110 |
| Number of reflections | 82062 | |
| <I/σ(I)> | 22.5 | 5.8 |
| Completeness [%] | 94.4 | 85.2 |
| Redundancy | 3.9 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | PROTEIN (10 MG/ML) MIXED WITH EQUIVALENT VOLUME OF WELL SOLUTION (150 MM NA CITRATE (PH 6.5), 700 MM AMMONIUM SULFATE) |
