2BSW
Crystal structure of a glyphosate-N-acetyltransferase obtained by DNA shuffling.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-12-12 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 69.323, 49.393, 46.492 |
| Unit cell angles | 90.00, 103.47, 90.00 |
Refinement procedure
| Resolution | 45.170 - 1.630 |
| R-factor | 0.181 |
| Rwork | 0.179 |
| R-free | 0.21300 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.360 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | CCP4 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.690 |
| High resolution limit [Å] | 1.630 | 1.630 |
| Rmerge | 0.070 | 0.160 |
| Number of reflections | 18939 | |
| <I/σ(I)> | 20.2 | 8 |
| Completeness [%] | 97.4 | 98.1 |
| Redundancy | 2.94 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 100 MM SODIUM ACETATE, PH 4.6 250 MM AMMONIUM SULFATE 25% PEG 4000 |
