2BSK
Crystal structure of the TIM9 Tim10 hexameric complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-ID-B |
| Synchrotron site | APS |
| Beamline | 14-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-05 |
| Detector | MARRESEARCH |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 107.430, 107.430, 110.588 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 16.000 - 3.300 |
| R-factor | 0.2679 |
| Rwork | 0.268 |
| R-free | 0.31930 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.440 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | RESOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.600 |
| High resolution limit [Å] | 3.450 | 3.450 |
| Rmerge | 0.080 | 0.370 |
| Number of reflections | 9357 | |
| <I/σ(I)> | 17 | 2.42 |
| Completeness [%] | 89.9 | 85 |
| Redundancy | 8.8 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M TRIS-HCL PH 8.5, 28% W/V PEG 3000, 0.2 M NACL |
