2BRW
Crystal structure of Streptococcus Pneumoniae Hyaluronate Lyase from 30percent PEGMME.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | OXFORD INSTRUMENTS |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 86.675, 84.061, 98.665 |
| Unit cell angles | 90.00, 98.87, 90.00 |
Refinement procedure
| Resolution | 95.350 - 2.800 |
| R-factor | 0.231 |
| Rwork | 0.229 |
| R-free | 0.27700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1egu |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.587 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 95.350 | 2.470 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.120 | 0.690 |
| Number of reflections | 45379 | |
| <I/σ(I)> | 7.6 | 2.2 |
| Completeness [%] | 87.3 | 78.2 |
| Redundancy | 2.8 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 30% POLYETHYLENE GLYCOL MONOMETHYL ETHER (PEGMME) 2000, 0.1 M MES BUFFER, PH 6.5 |
