2BRF
Crystal Structure of the FHA Domain of Human Polynucleotide Kinase 3' Phosphatase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-12-01 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.812, 40.047, 37.512 |
| Unit cell angles | 90.00, 90.96, 90.00 |
Refinement procedure
| Resolution | 37.500 - 1.400 |
| R-factor | 0.152 |
| Rwork | 0.150 |
| R-free | 0.18300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ujx |
| RMSD bond length | 0.030 |
| RMSD bond angle | 2.512 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.500 | 1.440 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.050 | 0.160 |
| Number of reflections | 16490 | |
| <I/σ(I)> | 8.9 | 3.9 |
| Completeness [%] | 97.2 | 88 |
| Redundancy | 3.4 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | PROTEIN WAS CRYSTALLISED IN 0.1 M TRIS-HCL PH 8.5, 2.0 M AMMONIUM SULPHATE |
