2BR2
RNase PH core of the archaeal exosome
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 206.880, 212.720, 434.050 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 218.220 - 2.800 |
| R-factor | 0.216 |
| Rwork | 0.215 |
| R-free | 0.23700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oyr |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.250 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 220.000 | 3.960 |
| High resolution limit [Å] | 2.790 | 2.790 |
| Rmerge | 0.160 | 0.620 |
| Number of reflections | 233146 | |
| <I/σ(I)> | 7.7 | 2 |
| Completeness [%] | 99.2 | 96 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 50 MM MES, 2.4 M SODIUM MALONATE, PH 6.5 |
