2BQZ
Crystal structure of a ternary complex of the human histone methyltransferase Pr-SET7 (also known as SET8)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 42.179, 46.322, 51.998 |
| Unit cell angles | 64.74, 86.66, 90.61 |
Refinement procedure
| Resolution | 20.000 - 1.500 |
| R-factor | 0.188 |
| Rwork | 0.187 |
| R-free | 0.20600 |
| Structure solution method | OTHER |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.579 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 19.900 |
| High resolution limit [Å] | 1.500 |
| Rmerge | 0.070 |
| Number of reflections | 63273 |
| <I/σ(I)> | 9.5 |
| Completeness [%] | 89.1 |
| Redundancy | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | pH 7.00 |
