2BQX

Inorganic Pyrophosphatase from the Pathogenic Bacterium Helicobacter pylori-Kinetic and Structural Properties

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL12B2
Synchrotron site	SPring-8
Beamline	BL12B2
Temperature [K]	100
Detector technology	CCD
Collection date	2003-12-12
Detector	ADSC CCD
Spacegroup name	P 63 2 2
Unit cell lengths	102.650, 102.650, 93.630
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.930 - 1.900
R-factor	0.217
Rwork	0.217
R-free	0.23200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qez
RMSD bond length	0.005
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.930	2.020
High resolution limit [Å]	1.900	1.900
Rmerge	0.100	0.360
Number of reflections	192336
Completeness [%]	99.4	99.4
Redundancy	8.1	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1