2BPQ
Anthranilate phosphoribosyltransferase (TrpD) from Mycobacterium tuberculosis (Apo structure)
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-04-30 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 80.874, 82.415, 117.520 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.680 - 1.900 |
| R-factor | 0.192 |
| Rwork | 0.191 |
| R-free | 0.23000 |
| Structure solution method | MAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.411 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.910 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Rmerge | 0.070 | 0.410 |
| Number of reflections | 68243 | |
| <I/σ(I)> | 30.3 | 4.1 |
| Completeness [%] | 99.2 | 99.5 |
| Redundancy | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 0.2 M CACODYLIC ACID PH6, 7% PEG 6000, 2% BENZAMIDINE HCL, pH 6.00 |
