2BP6
crystal Structure of pseudomonas aeruginosa lectin (PA-IIL) complexed with a-L-Galactopyranoside
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-03-17 |
Detector | ADSC CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 52.629, 72.790, 54.530 |
Unit cell angles | 90.00, 94.38, 90.00 |
Refinement procedure
Resolution | 19.430 - 1.500 |
R-factor | 0.143 |
Rwork | 0.143 |
R-free | 0.16600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1uzv |
RMSD bond length | 0.012 |
RMSD bond angle | 1.398 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.430 | 1.550 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.050 | 0.230 |
Number of reflections | 63528 | |
<I/σ(I)> | 10.35 | 3.08 |
Completeness [%] | 97.0 | 95.1 |
Redundancy | 3.82 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | TRIS HCL 0.1M, PH8.5, 1.75 M AMMONIUM SULFATE, pH 8.50 |