2BP5

MU2 ADAPTIN SUBUNIT (AP50) OF AP2 ADAPTOR (SECOND DOMAIN), COMPLEXED WITH NON-CANONICAL INTERNALIZATION PEPTIDE VEDYEQGLSG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX14.2
Synchrotron site	SRS
Beamline	PX14.2
Temperature [K]	100
Detector technology	CCD
Collection date	2002-10-30
Detector	ADSC CCD
Spacegroup name	P 64
Unit cell lengths	124.997, 124.997, 74.661
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	105.410 - 2.800
R-factor	0.195
Rwork	0.192
R-free	0.25100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bxx
RMSD bond length	0.022
RMSD bond angle	1.978
Data reduction software	MOSFLM
Data scaling software	SCALA
Refinement software	REFMAC (5.2.0009)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.600	2.950
High resolution limit [Å]	2.800	2.800
Rmerge	0.070	0.750
Number of reflections	16497
<I/σ(I)>	36	3.7
Completeness [%]	99.8	100
Redundancy	17.5	15.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.1	289	2.2M NACL, 0.4M NA/K PHOSPHATE PH 7.1, 10MM DTT, 0.1M MES, 15% GLYCEROL, 16 DEGREES