2BO4
Dissection of mannosylglycerate synthase: an archetypal mannosyltransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 261.148, 151.288, 153.240 |
| Unit cell angles | 90.00, 90.41, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.950 |
| R-factor | 0.177 |
| Rwork | 0.176 |
| R-free | 0.19200 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.232 |
| Data reduction software | HKL |
| Data scaling software | HKL |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.070 | 0.510 |
| Number of reflections | 432716 | |
| <I/σ(I)> | 15 | 1.6 |
| Completeness [%] | 97.0 | 90 |
| Redundancy | 3 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.3 M TRI-SODIUM CITRATE, 0.1 M SODIUM ACETATE TRIHYDRATE, PH 4.6, 40% (V/V) MPD |
