2BM9

cmcI-N160 in complex with SAM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-3
Synchrotron site	ESRF
Beamline	ID14-3
Temperature [K]	100
Collection date	2004-04-21
Spacegroup name	P 21 21 21
Unit cell lengths	92.805, 103.027, 182.024
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.050 - 2.940
R-factor	0.267
Rwork	0.264
R-free	0.32900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2bm8
RMSD bond length	0.011
RMSD bond angle	1.307
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	91.300	3.000
High resolution limit [Å]	2.900	2.900
Rmerge	0.090	0.570
Number of reflections	37586
<I/σ(I)>	14	2.2
Completeness [%]	99.9	100
Redundancy	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5		pH 7.50