2BM7

The Structure of MfpA (Rv3361c, P3221 Crystal form). The Pentapeptide Repeat Protein from Mycobacterium tuberculosis Folds as A Right- handed Quadrilateral Beta-helix.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	93
Detector technology	IMAGE PLATE
Detector	MSC RAXIS IV
Spacegroup name	P 32 2 1
Unit cell lengths	84.080, 84.080, 147.122
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.700
R-factor	0.229
Rwork	0.229
R-free	0.28900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PREVIOUS STRUCTURE DETERMINED BY SE-MET MAD
RMSD bond length	0.011
RMSD bond angle	1.600
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.030	0.180
Number of reflections	16076
<I/σ(I)>	22.5	6.3
Completeness [%]	94.0	90.5
Redundancy	4.5	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		4		PROTEIN (10MG/ML, 10% ETHYLENE GLYCOL, 200 MM AMMONIUM SULFATE) CRYSTALLIZED IN 30 % ETHYLENE GLYCOL, 100 MM CITRATE PHOSPHATE PH 4.0.