2BM0
Ribosomal elongation factor G (EF-G) Fusidic acid resistant mutant T84A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-04-30 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 78.200, 88.500, 116.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.400 |
| R-factor | 0.21 |
| Rwork | 0.210 |
| R-free | 0.27400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fnm |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.520 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.600 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.060 | |
| Number of reflections | 27567 | |
| <I/σ(I)> | 19.1 | 4.1 |
| Completeness [%] | 99.6 | 87.9 |
| Redundancy | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.3 | 17% PEG 8000 100 MM HEPES 46 MM TRIS-HCL PH 7.3 |
