2BLE
Structure of human guanosine monophosphate reductase GMPR1 in complex with GMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-09 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 117.780, 117.780, 54.420 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.900 |
| R-factor | 0.18 |
| Rwork | 0.177 |
| R-free | 0.22800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1eep |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.663 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.120 | 0.460 |
| Number of reflections | 27431 | |
| <I/σ(I)> | 27.1 | 2.11 |
| Completeness [%] | 89.1 | 78.7 |
| Redundancy | 7.6 | 4.04 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.1 NA-CACODYLATE PH=6.5 17% PEG10K 0.2 M CA-ACETATE 25% GLYCEROL, pH 6.50 |
