2BL2
The membrane rotor of the V-type ATPase from Enterococcus hirae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-23 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 120.136, 125.604, 210.866 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 61.000 - 2.100 |
| R-factor | 0.191 |
| Rwork | 0.190 |
| R-free | 0.20000 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.955 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.300 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.090 | 0.540 |
| Number of reflections | 184567 | |
| <I/σ(I)> | 9.1 | 1.8 |
| Completeness [%] | 99.4 | 97.5 |
| Redundancy | 3.7 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 32% PEG 400, 220MM NA CITRATE, 0.32MM DODECYLMALTOSIDE, 1.2MM UNDECYLMALTOSIDE, pH 8.00 |
