2BKV
Structure and kinetics of a monomeric glucosamine-6-phosphate deaminase: missing link of the NagB superfamily
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 120 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.009, 48.023, 71.761 |
| Unit cell angles | 90.00, 91.04, 90.00 |
Refinement procedure
| Resolution | 72.550 - 1.500 |
| R-factor | 0.167 |
| Rwork | 0.165 |
| R-free | 0.21100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB EBTRY 1DEA |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.698 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.140 | 0.430 |
| Number of reflections | 67231 | |
| <I/σ(I)> | 14.6 | 4.6 |
| Completeness [%] | 98.8 | 97.7 |
| Redundancy | 5.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
