2BKP
STRUCTURE ANALYSIS OF UNKNOWN FUNCTION PROTEIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-10-23 |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 71.052, 71.052, 98.180 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.410 - 2.200 |
| R-factor | 0.225 |
| Rwork | 0.225 |
| R-free | 0.29000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vct |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.090 | 0.430 |
| Number of reflections | 13395 | |
| <I/σ(I)> | 32.1 | 5.4 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 13.4 | 13.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.7 | PROTEIN WAS CRYSTALLIZED FROM 1.25 M NACL, 50 MM ACETATE, PH 4.7; THEN SOAKED WITH 1.5 M KCL. |
