2BK8
M1 domain from titin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-16 |
| Detector | MARRESEARCH |
| Spacegroup name | I 41 |
| Unit cell lengths | 68.780, 68.780, 48.180 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 17.740 - 1.690 |
| R-factor | 0.191 |
| Rwork | 0.189 |
| R-free | 0.23400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | HOMOLOGY MODEL |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.393 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.740 | 1.800 |
| High resolution limit [Å] | 1.690 | 1.690 |
| Rmerge | 0.040 | 0.410 |
| Number of reflections | 24619 | |
| <I/σ(I)> | 18.1 | 3.89 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 2.2 M AMMONIUM SULFATE 0.1 M BIS TRIS PH 7.0 |
