2BJE
Acylphosphatase from Sulfolobus solfataricus. Monclinic P21 space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.980, 56.532, 60.805 |
| Unit cell angles | 90.00, 103.69, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.900 |
| R-factor | 0.166 |
| Rwork | 0.162 |
| R-free | 0.21500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2acy |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.997 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.540 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.080 | 0.330 |
| Number of reflections | 25338 | |
| <I/σ(I)> | 9.7 | 2.9 |
| Completeness [%] | 98.3 | 95.7 |
| Redundancy | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
